Trehalose facilitates DNA melting: a single-molecule optical tweezers study

Bezrukavnikov, S.; Mashaghi, A.; van Wijk, R. J.; Gu, C.; Yang, L. J.; Gao, Y. Q.; Tans, S. J.
Abstract:
Using optical tweezers, here we show that the overstretching transition force of double-stranded DNA (dsDNA) is lowered significantly by the addition of the disaccharide trehalose as well as certain polyol osmolytes. This effect is found to depend linearly on the logarithm of the trehalose concentration. We propose an entropic driving mechanism for the experimentally observed destabilization of dsDNA that is rooted in the higher affinity of the DNA bases for trehalose than for water, which promotes base exposure and DNA melting. Molecular dynamics simulation reveals the direct interaction of trehalose with nucleobases. Experiments with other osmolytes confirm that the extent of dsDNA destabilization is governed by the ratio between polar and apolar fractions of an osmolyte.
Year:
2014
Type of Publication:
Article
Journal:
Soft Matter
Volume:
10
Number:
37
Pages:
7269-7277
Month:
October
Note:
[DOI:\href{https://dx.doi.org/10.1039/c4sm01532k}{10.1039/c4sm01532k}] [PubMed:\href{https://www.ncbi.nlm.nih.gov/pubmed/25096217}{25096217}]
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